Middle School Biology
What you'll learn
In this 2-day biology course, students will learn lab safety and proper use of equipment, how to use an autoclave to sterilize bacterial culture media, how to make petri dishes with agar for bacterial culture, and how to inoculate GFP plasmid for overnight growth. They will be performing a GFP Plasmid mini prep, running bacterial cells through a centrifuge, and will be isolating and purifying plasmid DNA. They will make and run agarose gel electrophoresis for confirmation of DNA isolation and view their results on a trans-illuminator.
Course Includes
Day 1
First hour lab safety and using equipment. Autoclave- sterilizing bacterial culture media. Making of petri dishes with agar for bacterial culture. Inoculating GFP plasmid for overnight growth.
Day 2
GFP Plasmid miniprep. Centrifuge bacterial cells, isolate and purify plasmid DNA. Make and run agarose gel electrophoresis for conformation of DNA isolation and view on UV-Vis tran-illuminator.
Classes Offered
Choose 1 option
Monday June 27 2pm-4pm
Tuesday June 28 2pm-4pm
Monday August 8 10am-12pm
Tuesday August 9 10am-12pm
Wednesday August 10 10am-12pm
Thursday August 11 10am-12pm
Monday August 22 2pm-4pm
Tuesday August 23 2pm-4pm
Wednesday August 24 2pm-4pm
Thursday August 25 2pm-4pm